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Immunodiagnosis of Fasciolosis in horses and pigs using Western blots

Universidad de Chile. Repositorio Académico

Título Immunodiagnosis of Fasciolosis in horses and pigs using Western blots
Autor Aballay Delgado, Jimena Margarita
Fredes Martínez, Fernando Guillermo
Aguillón Gutiérrez, Juan Carlos
Silva Sandoval, Marco Antonio
Alcaíno Contador, Héctor A.
Gorman Goffreri, Texia
Tema Fasciolosis
Descripción Artículo de publicación ISI
Crude and partially purified somatic (S) and excretorysecretory (ES) antigens of Fasciola hepatica were
subjected to Western blot analysis in order to identify polypeptides that would enable specitic and sensitive
immunodiagnosis of horse and pig fasciolosis to be undertaken. Sera from 20 horses and 20 pigs with natural
infections of F. hepatica and the same number of uninfected hosts of each species were tested, together with
sera from 2 pigs with Cysticercus cellufosae infections. Using crude S antigens, sera from infected horses and
pigs reacted specifically with a wide range of polypeptides of 14-19, 22-30, 35-37 and 42KDa. Likewise,
specific reactivity between polypeptides of 14-17, 22-30 and 40 - 42 kDa in crude ES antigens and sera from
infected horses and pigs was obtained. Against the criteria of high sensitivity and speciticity, the 22-30-kDa
polypeptides would appear to be the most suitable candidate antigens for use in the immunodiagnosis of
fasciolosis in horses and pigs. 0 1997 Australian Society for Parasitology. Published by Elsevier Science Ltd.
FONDECYT (Pr. No. 194 - 0245).
Fecha 2014-10-15T18:34:40Z
Tipo Artículo de revista
Enlace International Journal for Parasitology, 27:1 (1429) 1997
Idioma en_US
Derechos Attribution-NonCommercial-NoDerivs 3.0 Chile
Publicador Pergamon